ACE columns are manufactured by Advanced Chromatography Technologies (ACT) and launched into the market by 2000. The key criteria in analytical chromatography are selectivity and batch to batch column reproducibility. Since its beginning ACE invests substantial amount of funds every year into research to stay ahead of the rest with regards to column selectivity and reproducibility.
ACE Chromatography materials are based on new generation ultra pure base deactivated silica available in a wide range of bonding chemistries and particle sizes.
Dont Miss this Deal: Chose one Method Development Kit and pay only for one column in the kit!
ACE Product Classes
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The ACE product range comprises of:
- ACE UltraCore SuperC18
- ACE Ultra Core SuperPhenylHexyl
- ACE Excel CN-ES
- ACE Excel C18-Amide
- ACE Excel SUPERC18
- ACE Reverse Phase Columns (C18, C8, C4
- ACE Polar Bonded Columns (CN
- ACE Silica – Normal Phase Columns (SIL)
- ACE Phenyl Bonded Phase Columns (Phenyl)
- ACE Highly Aqueous Reverse Phase Columns (AQ , C18-AR)
- ACE Fluorinated Columns (C18-PFP)
- ACE High Load Column(C18-HL)
- ACE Wide Pore (Biochromatography ) Column
- ACE Column Kits
- ACE LC-MS and Rapid Analysis Columns
- ACE Capillary and Nano-Column
- ACE Prep Column
ACE EXCEL Product Range for uHPLC and SCF chromatography
- ACE Excel Column ( C18, C18-AR, C18-PFP, C8, C4, CN, Phenyl, AQ, SIL, C18 HL)
New Products
ACE Ultra Core Super C18 & ACE Ultra Core SuperPhenyl Hexyl
ACE Excel CN-ES for UHPLC and HPLC
Provides very strong dipole-dipole and hydrophobic binding interaction
Target Analytes: Polar and non-polar analytes, Analytes with double and tripple bonds, Analytes differing in hydrophobicity, Polar molecules having insufficient retention on traditional CN bonded phases.
Recomended Separations: Mixtures of very polar, polar and non-polar analytes, uitable for NP and RP separation, separations where traditional CN bonded phases show insufficient stability/lifetime, applications where a typical C18 column does not provide adequate separations, as an orthogonal phase in method development.
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ACE Excel C18-Amide .
Is a polar embedded phase with a uniquely engineered ligand for low to medium pH range applications, multi-mode separations and method development. 100% Water can be used. Analyst in the Food and beverage industry will be surprised what can be achieved with ACE C18-Amide
Ideal column for method development: Alternative selectvity to C18 and C8 columns with polar molecules, especially for acids, compatible with 100% aequeous mobile phases , high efficiency 2 um, 3um, 5 um and 10 um particles for UHPLC, HPLC and preparative separations, low bleed for UV and LC/MS compatibility.
Recommended Application: Small water soluble analytes and polar compounds, H-bond donors, acids, bases and phenolic compounds
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ACE Excel SUPERC18
The ACE SuperC18 is available in 2 um, 3 um, 5 um, 10 um particle sizes. It covers UHPLC, HPLC, SCFC and semi-prep column dimensions.
- Exploit selectivity at low, intermediate and high pH
- Use with LC/MS compatible buffers between pH 1.5 – 11.5
- High efficiency 2μm, 3μm, 5μm and 10μm particles for UHPLC and HPLC
- Ultra-inert for maximum performance and reproducibility
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General Product Benefits
- Excellent performance : up to 200,000 plates / metre
- ACE & ACE Excel are extremely popular column in the market
- Column lengths : 1 cm – 25 cm
- Column diameters: 1.0 mm – 30.0 mm
- Particle sizes ACE: 3 µm, 5µm, 10 µm and 15µm
- Particle sizes ACE Excel: 2 µm , 3 µm, 5µm
- Pore sizes: 100 Å and 300 Å
- Range of bonded phases : C18, C8, C4, CN, Phenyl, AQ, C18-HL, C18-AR, C18-PFP
- Fully validated Columns
- Excellent for LC/MS and rapid analysis applications
- The ultra inert characteristics of all ACE phases make them the ideal choice for separating polar basic compounds.
- ACE columns consistently produce outstanding peak shape and column efficiency when separating troublesome basic compounds.
- ACE High Purity Base Deactivated Silica guarantee reproducibility, excellent column lifetime and excellent performance with acidic, basic and neutral molecules by the most stringent of validation protocols.
- Independent comparison tests show ACE HPLC columns give outstanding performance. I
How to order:
- If you know your column specification please sent it to us so that we can send you a written quotation
- If you are a newcomer please send us specification of your compound or compound category to be separated and preferred. process conditions.
- If you have advanced knowledge in chromatographic method development please consult the product data below or download a ACE product catalogue. Send us your specification for quotation
Separation of small molecules (MW < 2000)
ACE Particles with 100 A Pore sizes
- ACE bonded phases are available in 3, 5, 10 and 15µm particle sizes, so regardless of the application, the method can be scaled up or scaled down and the same selectivity be assured. For fast, high resolution preparative chromatography, a range of pre-packed ACE preparative and combinatorial chemistry columns are available.
- Method development is recommended to start with C18 or C8, knowing that if more retention and hence more resolution is needed, the next choice is C18-HL (Hi-Load). Starting with C8 offers the benefit of shorter analysis times and/or lower organic solvent use. The elution order for most compounds will be the same on the aliphatic (C18, C8, C4) phases. If a different elution order is required for compound verification or to resolve matrix components, changing to a phenyl or CN phase is simpler than trying to change selectivity by eluent changes. In many cases, the ACE CN and ACE Phenyl phases will offer a significant difference in selectivity from the aliphatic phases.
- Consider also ACE LC-MS and rapid analysis columns .
- Scale up or Scale down
Separation of large molecules (MW > 2000 – 60 kDa)
ACE 300 A Material
Reverse Phase Columns
ACE C18 Columns
Description:
- Optimised for maximum efficiency,
- superior peak shape and resolution. Utilises the same ultra high purity silica as all ACE phases.
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Application:
- Recommended starting point for method development.
- Also suited to high aqueous conditions and for rapid analysis applications.
- A C18 phase for most HPLC applications.
- Available in a range of particle sizes,
- From LC-MS and microbore applications through to preparative scale separations
ACE C8 Columns
Description
- Increased bonding density compared to ACE C18.
- Similarly optimised for maximum efficiency, superior peak shape and resolution.
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Application:
Recommended starting point for method development. Also suited to high aqueous conditions and for rapid analysis applications.
ACE C4 Columns
Description
- Combines lower hydrophobicity with excellent chromatographic performance. Improved hydrolytic stability compared to conventional C4 phases.
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Application:
- Use for rapid analysis optimisation, when less retention than C8 or C18 is required.
- Also suitable for analysis of small proteins.
Polar Bonded Phases / Columns
ACE CN Columns
Description:
- Suitable for use in both normal- and reversed phase modes.
- Greatly improved performance, stability and reproducibility compared to conventional CN phases
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Applcations
- Use to increase retention of polar compounds.
- Ideal for gradients and rapid screening applications due to fast equilibration capacity
ACE SIL Columns
Description
- Non-bonded silica for use in normal-phase mode
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Application
- Useful for separation of isomeric compounds.Specialty Products
Phenyl bonded Phases
ACE Ph Columns
Description
- Hydrophobicity between C4 and C8 phases, with increased polar selectivity.
- Improved performance, stability and reproducibility compared to conventional phenyl phases.
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Application
- Use for rapid analysis optimisation, when less retention than C8 or C18 is required.
- Also suitable for analysis of small proteins.
High Aequeous RP Phases
Description
- A C18 bonded phase with integral polar functionality
- Retains polar compounds in 100% aqueous eluent
- Unique C18 bonded phase with integral polar functionality
- Resistant to retention loss in 100% aqueous eluent
- Rapid gradient re-equilibration (0-100%)
- Excellent peak shape with acidic, basic and neutral molecules
- Ideal for LC-MS applications
- Unmatched reproducibility
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Application:
- Recommended for applications where 100% aqueous eluents are required
- ACE AQ is an ultra-inert phase designed for the reversed-phase separation of very polar compounds with up to 100% aqueous eluent. ACE AQ is ideal for use with fast gradients due to its rapid re-equilibration proper Maximum Reproducibility in High Aqueous Eluents ACE AQ is particularly recommended for applications requiring high aqueous content eluents. Improved resistance to phase collapse is seen compared to standard C18 phases.
- When separating very polar, water-soluble compounds, highly aqueous (>95%) eluents are often required to achieve sufficient retention. However, operating a conventional C18 column under such conditions can lead to poor chromatographic reproducibility. Over time peaks will elute with shorter and shorter retention times and resolution between peaks will deteriorate.
- ACE AQ columns introduce an integral polar functionality, which prevents this retention loss when using highly aqueous mobile phases.rties and resistance to retention loss.
- Increased Resolution with 3µm Columns
- With today’s increased pace of drug discovery, fast and efficient methods are required. Short, narrow-bore columns are replacing the conventional 250 x 4.6mm versions. ACE HPLC columns are available in both 3 and 5µm particle sizes. Although 5µm particles are sufficient for most applications, greater efficiency can be obtained by using smaller particles. This increased efficiency enables the use of shorter (<150mm) column lengths, resulting in decreased analysis times. Due to the excellent flow characteristics of ACE silica, the high back pressures often encountered with other columns are eliminated.
ACE C18-AR Columns
Discription
- ACE C18-AR: a C18 bonded phase with unique selectivity
- Alternate selectivity to “standard” C18 columns – additionally recommended for compounds with aromatic functionality
- Ultra inert, ultra high purity silica, for excellent peak shape and reproducibility
- Compatible with highly aqueous mobile phases to enable the retention and separation of polar compounds
- Exceptional bonded phase stability for elevated temperature applications
- Availability in high throughput column dimension
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Application
- Improved chromatographic resolution
ACE Fluorinated Phases
ACE C18-PFP
Description
- ACE C18-PFP: a C18 bonded phase with extra resolving poweh
- Combines C18 & PFP mechanisms of separation to separate mixtures not possible with either phase alone
- Ultra inert, ultra high purity silica, for excellent peak shape and reproducibility
- Exceptional bonded phase stability for elevated temperature applications
- Ultra low bleed phase ensures UV and LC/MS compatibility
- Availability in high throughput column dimensions
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Application
- Useful for separation of isomeric compounds
ACE High Load Phases / Columns
ACE C18-HL (High-Load)
Description
- Higher carbon load than ACE C18 leading to increased retention times and higher loadability.
- High surface area, high carbon load phase
- 3, 5, 10 and 15µm particle sizes
- Increased loading and retention
- Exceptional chemical stability
- Excellent peak shape with acidic, basic and applications neutral molecules
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Application
- Availability of particle sizes up to 15µm ensures easy scale-up for preparative and process scale applications.
- Optimised for preparative and process scale
- The increased retention characteristics of ACE C18-HL make it an ideal selection for LC-MS applications. Retention can be maintained whilst reducing the aqueous content of the eluent thus increasing sensitivity, .
- For preparative applications, the higher surface area leads to increased loading capacity. Available in 3, 5, 10 and 15µm particle sizes and a wide range of column dimensions. ACE C18-HL columns show reproducible scale up from LC-MS to preparative dimensions
Wide Pore RP Phases for separation of large molecules (MW 2 – 40 KDa)
ACE 300A Columns for Biotechnology Application
Benefits:
- 300 A ultra high purity silica
- Up to 50 KDa MW large molecules
- Ultimate peptide and protein application column
- C18, C8, C4, CN and Phenyl chemistries
- 3.5. and 10 um particle sizes
- Unmatched reproducibility
- Exceptional chemical stability
ACE C18 – 300
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Application: Peptides
ACE C 8 – 300
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Application : Peptides and Proteins
ACE C4 – 300
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Applications: Peptides and Proteins
ACE CN – 300
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Applcations
ACE Ph – 300
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Application
ACE Column Kits
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LC-MS and Rapid Analysis Columns
Feature
- High performance – excellent peak shape for higher sensitivity
- Choice of 13 low bleed phases for complete optimization
- Ultra-inert silica enables MS compatible buffers to be used
- 20, 30, 35 and 50 mm column lengths
- 1.0, 2.1, 3.0, 4.0 and 4.6 mm i.d.s
- Nowhere is the need for a truly ultra-inert base deactivated HPLC column more important than in high-throughput analysis or LC-MS. Even subtle differences in silanol activity between columns can markedly affect the chromatography in the very short, fast columns typically used. In addition, any peak tailing due to silanol activity can have a profound effect on detection limits in high sensitivity assays.
- ACE ultra-inert base deactivated HPLC columns virtually eliminate the negative effects of silanols in HPLC separations. This unequalled performance is now available in 20, 30, 35 and 50mm length columns, with diameters from 1.0 to 4.6mm. The ACE columns are suitable for high throughput and LC-MS applications, and are the ideal choice for high volume screening assays used for drug analysis and combinatorial libraries where robust, reproducible columns are essential.
- Increased Column Robustness
- ACE LC-MS and rapid analysis columns are manufactured by a unique process which results in increased column bed stability, thus increasing column lifetime.
- LC-MS Buffer Compatibility
- ACE LC-MS columns have an extremely low requirement for buffer salts or modifiers in order to achieve good peak shape. ACE columns have been demonstrated to be extremely robust at high and low pH. This broad pH operating range enables thorough optimization of pH and simplifies method development to a single column rather than selecting 3 different columns with low, intermediate and high pH stability.
- For maximum LC-MS compatibility under acidic conditions organic acids such as formic, acetic and TFA are recommended. Under basic conditions ammonium bicarbonate, ammonium acetate and ammonium hydroxide buffers are recommended.
ACE Capillary and Nano Columns
- High sensitivity
- Low sample mass and volume application
- Compatible with LC-MS and LC-MS/MS
- Very low solvent consumption
- Suitable for proteomics
- Compatible with LC-MS and LC-MS/MS
Brochure: ACE_Nano-and-LCMS-Columns
ACE Capillary Column
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ACE Prep- HPLC Column
ACE preparative HPLC columns are available in a wide range of column dimensions and particle sizes for complete optimization of preparative assay
Benefits:
- Get High Purity Product Fast
- Ultra high purity base deactivated silica
- 5, 10 and 15 um particles
- Fully validated column
- Exceptional reproducibility
- Excellent efficiencies
- High sample recovery
- Excellent column lifetime
- 100 A and 300 A pore-sizes
For maximum loadability, 30mm or 50mm i.d. columns are recommended. Use a 50mm length ‘combinatorial’ column with a 5μm particle size to maximize the speed of your analysis. To maximize resolution, choose a 250mm length column with a 5μm particle size
Loading specifications.
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Increased Preparative Column Lifetimes
A fundamental requirement for successful preparative applications is a robust, rugged column. ACE materials have previously been shown to have excellent chemical stability under highly acidic and highly basic conditions. The high mechanical stability of the ultra-inert ACE silica and the unique preparative packing methods employed result in a high efficiency, extremely stable packed silica bed – leading to increased preparative column lifetimes. The following figure demonstrates the excellent column performance maintained with a 50 x 21.2mm ACE 5 AQ column over an extended test period.
ACE EXCEL Columns
ACE Excel is a ultra high column pressure system available in a three different particle diameter, all the well proven ACE surface chemistry packed in a wide range of cartridges.
ACE® Excel UHPLC columns offer:-
- Ultra robust columns – up to 1000 bar (15000 psi)
- Unique phases for rapid method development
- Available with high efficiency 2 µm, 3 µm and 5 µm particle sizes
- Compatible with all UHPLC and HPLC Systems
Custom design your own ACE Excel UHPLC colum as follows:
1. Decide which surface chemistry and particle size you require.
2. Make a note of the resultant EXL number: e.g EXL-101 for 2 um C18
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3. Add beside the EXL number the Cartridge dimension number and click here to send us your request
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