HPLC Columns Equivalent selection guide
SEC (aqueous GFC): PROTEIN KW |
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Shodex | Tosoh | Phenomenex | GE HealthCare | Bio-Rad | Sepax | |
8.0 × 300 mm | 7.8 × 300 mm | 7.8 × 300 mm | 3.2 × 300 mm | 7.8 × 300 mm | 7.8 × 300 mm | |
PROTEIN KW-802.5 (150 Å, 5 μm, 5.000-150.000) |
TSKgel G2000SWxl |
BioSep SEC-s2000 |
Superdex 75 PC (13 μm) |
Bio-Sil SEC 125-5 |
SRT SEC-100, SRT SEC 150 | |
PROTEIN KW-803 (300 Å, 5 μm, 10.000 - 700.000) |
TSKgel G3000SWxl |
BioSep SEC-s3000 |
Superdex 200 PC (13 μm) |
Bio-Sil SEC 250-5 |
SRT SEC-300 | |
PROTEIN KW-804 (500 Å, 7 μm, 10.000- 1.000.000) |
TSKgel G4000SWxl |
BioSep SEC-s4000 |
none |
Bio-Sil SEC 400-5 |
SRT SEC-500 | |
PROTEIN KW-G (6.0 x 50 mm) |
TSKgel Guard SWxl |
Guard holder |
|
Bio-Sil SEC Guard |
SRT SEC Guard | |
SEC (aqueous GFC): OHpak SB-800 |
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Shodex | Tosoh | Phenomenex | Agilent (PL) | Waters | Sepax | Thermo Scientific | Jordi Labs | |
8.0 × 300 mm | 7.8 × 300 mm | 7.8 × 300 mm | 7.5 × 300 mm | 7.8 × 300 mm | 7.8 × 300 mm | 7.8 × 300 mm | 7.8 x 300 mm | |
OHpak SB-802.5 HQ (100 -10.000) | TSKgel G3000PWxl | PolySep-SEC GFC-P 2000 | PL aquagel-OH 30 | Ultrahydrogel 250 | SRT-C SEC-100 | Acclaim SEC-300 | Jordi Gel DVB Glucose100 Å | |
OHpak SB-803 HQ (1.000 -100.000) | TSKgel G4000PWxl | PolySep-SEC GFC-P 3000 | PL aquagel-OH 40 | Ultrahydrogel 500 | SRT-C SEC-150 | none | Jordi Gel DVB Glucose 500 Å | |
OHpak SB-805 HQ (10.000 -4.000.000) | none | PolySep-SEC GFC-P 5000 | none | none | SRT-C SEC-500 | none | Jordi Gel DVB Glucose 10.000 Å | |
OHpak SB-806M HQ (100 -20.000.000) | TSKgel GMPWxl | PolySep-SEC GFC-P Linear | PL aquagel-OH MIXED-H | Ultrahydrogel Linear | none | none | Jordi Gel DVB Glucose Mixed Bed | |
OHpak SB-G (6.0 x 50 mm) | TSKgel PWxl Guard | PolySep-SEC GFC-P Guard | PL aquagel-OH Guard | Ultrahydrogel Guard | none | none | Jordi Gel DVB Glucose Guard | |
SEC (organic solvent): GPC | ||||||
Shodex | Tosoh | Agilent (PL) | Waters | Jordi Labs | PSS |
|
8.0 × 300 mm | 7.8 × 300 mm | 7.5 × 300 mm | 7.8 x 300 mm | 7.8 x 300 mm |
|
|
GPC KF-802.5 | TSKgel G2500Hxl | PLgel 500 Å 5 μm | Styragel HR 2 | Jordi Gel DVB 500 Å |
|
|
GPC KF-803 | TSKgel G3000Hxl | PLgel 103 Å 5 μm | Styragel HR 3 | Jordi Gel DVB 1.000 Å |
|
|
GPC KF-804 | TSKgel G4000Hxl | PLgel 104Å 5 μm | Styragel HR 4 | Jordi Gel DVB 10.000 Å |
|
|
GPC KF-805 | TSKgel G5000Hxl | PLgel 105Å 5 μm | Styragel HR 5 | Jordi Gel DVB 100.000 Å |
|
|
GPC KF-806 | TSKgel G6000Hxl | PLgel 106Å 10 μm | Styragel HR 6 |
|
|
|
SUGAR (Ligand exchange) | ||||||||||
separation mode | analytes | Shodex | USP list | Bio-Rad | Agilent (PL) | Phenomenex | Waters | Sepax | Supelco/Sigma-Aldrich |
|
|
| 8.0 x 300 mm |
| 7.8 x 300 mm | 7.7 x 300 mm | 7.8 x 300 mm | 6.5 x 300 mm | 7.8 x 300 mm | 7.8 x 300 mm |
|
ligand exchange + size exclusion | mono-, disaccharides | SUGAR SC1011 (Ca2+) | L19, L22 | Aminex HPX-87C (Ca2+) | Hi-Plex PL Ca | Rezex RCM-Monosaccharide Ca+2 | Sugar-Pak I | Carbomix Ca | Supelcogel Ca |
|
ligand exchange + size exclusion | mono-, disaccharides | SUGAR SP0810 (Pb2+) | L22, L34 | Aminex HPX-87P (Pb2+) | Hi-Plex PL Pb | Rezex RPM-Monosaccharide Pb+2 | none | Carbomix Pb | Supelcogel Pb |
|
ligand exchange + size exclusion | mono-, disaccharides | SUGAR KS-801 (Na+) | L22, L58 | Aminex HPX-87N (Na+) | Hi-Plex PL Na | Rezex RNM-Carbohydrate Na+ | none | Carbomix Na | none |
|
ion exclusion + size exclusion | sugars + organic acids | SUGAR SH1011 (H+) | L17, L22 | Aminex HPX-87H (H+) | Hi-Plex PL H | Rezex RHM-Monosaccharide H+ | none | Carbomix H | Supelcogel H |
|
ion exclusion | organic acids | RSpak KC-811 (H+) | L17, L22 | none | none | Rezex ROA-Organic Acid H+ | none | none | none |
|
Reversed Phase (RP): C18 | ||||||||
Shodex | Shodex | Shodex | Agilent | Waters | Merck | Phenomenex | Kromasil/Akzo Nobel | Supelco/Sigma-Aldrich |
Silica (USP L1) | Polymer | Polymer | Silica | Silica | Silica | Silica | Silica | Silica |
Silica C18, Silica C18M, Silica C18P |
Asahipak ODP-40, Asahipak ODP-50 |
ODP2 HP |
Zorbax ODS C-18, MetaSIL C18, OmniSpher C18, Polaris C18, Pursuit C18 | Spherisorb ODS, SunFire C18, Symmetry C18, Atlantis T3/dC18, Xterra RP18, µBondapak C18 |
LiChrospher RP-18, LiChrosorb RP-18, Superspher RP-18, Purospher RP-18 | Kinetex C18, Gemini C18, Luna C18, Jupiter C18, Prodigy C18, Aqua C18, Synergi Fusion- RP/Hydro-RP |
Kromasil C18, Eternity C18 |
Ascentis C18, SupelcoSIL LC- 18, |
Thermo Fisher/Dionex | Machery-Nagel | Hichrom | GL Sciences | Restek | Shiseido | |||
Silica | Silica | Silica | Silica | Silica | Silica | |||
Acclaim 120 C18, Hypersil ODS (C18), BetaSil C18 |
Nucleosil C18, Nucleodur C18 |
Partisil ODS |
Inertsil ODS | Pinnacle C18, Viva C18, Allure C18, Ultra C18 |
Capcell Pak C18 | |||
other dimensions available, please compare particle size and pore size |
| |||||||
HILIC: Asahipak NH2P (Amino) | |||||||||||
Shodex | Sepax | Tosoh | Agilent | Waters | Kromasil/Akzo Nobel | Supelco/Sigma-Aldrich | Merck | Restek | YMC | Jordi Labs |
|
Asahipak NH2P (polymer- based) |
HP-Amino |
TSKgel NH2-100 |
ZORBAX NH2 |
Spherisorb Amino (NH2) |
Kromasil NH2 |
SUPELCOSIL LC-NH2 | LiChrospher NH2, Purospher STAR NH2 | Pinnacle II Amino, Hypersil APS 2 Amino |
YMC-Pack NH2 | DVB PolyAmino (polymer- based) |
|
on Wed, 07/04/2018 - 07:12 admin
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